CLCD is updated monthly, usually by mid-month, with an average of 1500 new reviews and MARC records.
Now that KLIATT, which has been a part of CLCD for several years, has decided to end its publication, CLCD is pleased to offer KLIATT subscribers an opportunity to try CLCD for two months at no charge.
This study was conducted to screen those newly released cotton cultivars for CLCD by conventional methods (field evaluation, whitefly-transmission, and grafting of the material) and by molecular diagnostic tools (multiplex PCR and dot blot hybridization).
In the field screening trials, S-12 was found to be the most susceptible to CLCD followed by CIM-240, MNH-93, BH-36, and NIAB-Karishma (Table 2).
CLCD is associated with four distinct begomoviruses and novel components: DNA-1 and DNA-beta (Zhou et al.
41% was found among the elite Pakistani cotton cultivars that mostly had been released before the CLCD epidemic (Iqbal et al.
In the present study, the multiplex PCR and dot-blot hybridization were found to be the most sensitive and refined diagnostic tools for screening the breeding material against the CLCD.
Abbreviations: CLCD, cotton leaf curl virus disease; RAPD, random amplified polymorphic DNA; RFLP, restriction fragment length polymorphism; PCR, polymerase chain reaction.