aureus investigational vaccine group, Pfizer, has already designed vaccine by addressing virulence mechanisms associated with CP5, CP8, ClfA
and MntC and declared that anti-ClfA antibodies can completely inhibit S.
aureus isolates carried at least two virulence genes and almost all the isolates contained clfa
(100%, 112/112) and hla (99.1%, 111/112) genes.
Interestingly, amplification using the four primer pairs of clfA
, clfB, sdr and spa were able to distinguish the tested strains according to their corresponding PFGE pulsotypes (sensitivity, specificity, PPV and NPV = 100%) (Figure 1).
reports that the members of its association provided a total of CZK63.68bn.
Caracterizacion molecular de aislamientos representativos de los clones prevalentes Origen/denominacion SAMR-CA Portacion SAMR-HA CAA A2 A1 Bra Cord Ped ST 5 5 5 239 5 5 SCCmec IV IV IV III I IV agr II II II I II II ACME - - - - - - sea + - + - - - seb - - - - - - sec - - - - - - sed - - - - - - see - - - - - - seg + + + - - + seh - - - - - - sei + + + + + + sej - - - - - - hlg + + + + + + luk-PV + - + - - - ica + + + + + + fnbA + + + + + + fnbB - - - + - - fib + + + + + + clfA
+ + + + + + clfB + + + + - + SAMR-HA: Staphylococcus aureus resistente a meticilina adquirido en el hospital SAMR-CA: Staphylococcus aureus resistente a meticilina adquirido en la comunidad.
The genome of the MRSA isolate encoded three hemolysin genes (hlb, hld and hlg2) and five adhesion genes (clfA
, clfB, fnbA, efb and icaA) (Fig.
(10) Moreover, all the isolates were submitted to PCR (11) for detection of virulence factor genes: sea (Staphylococcal enterotoxins type A), seb (Staphylococcal enterotoxins type B), sec (Staphylococcal enterotoxins type C), PVL (Panton-Valentine Leukocidin), ClfA
(clumping factor) and Spa (IgG-binding region and the X-region of protein A).