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Principal components analysis (PCA) was used to visualise effects of soil order and treatment on CLPPs (R Core Team 2009).
All three treatments differed significantly in CLPPs (Table 3, Fig.
This was also reflected in the CLPPs, whereby changes in urine-treated soils were likely driven by increased utilisation of urea and yeast extract and a decrease in the utilisation of arabinose.
We assessed the effect of bovine urine on functional capacity, functional diversity and CLPPs in a range of soils from intensive dairy grazing land use.
We found support for our hypothesis that urine addition decreases soil functional capacity, functional diversity and shifts CLPPs of microbial communities.
We also found that soil type had a greater effect on CLPPs than treatment, explaining 16-22% of the variation between sites, consistent with previous findings that soil type is a pivotal factor in the determining microbial parameters (Girvan et al.
In conclusion, we observed reduced functional capacity and diversity and a shift in CLPPs at 21 days after urine addition in a range of soil types.
Chapman SJ, Campbell CD, Artz RRE (2007) Assessing CLPPs using MicroResp[TM]: A comparison with Biolog and multi-SIR.
Caption: Figure 6: Ordination diagrams of CLPPs from principal component analyses of carbon utilization profiles of lake and river samples from different sites (a) and different seasons (b).
One commonly used approach in environmental microbiology is the community-level physiological profiling (CLPP) using Biolog EcoPlates, which is based on assessing the ability of microbial communities to metabolize a broad range of organic substrates .
The main goal of this study was to determine the potential of CLPP assay for resolving the metabolic diversity of river and lake sediments on temporal and spatial scales and to establish a close linkage between physicochemical properties of the sediments and the associated microbial functional activities.
Despite the differences in the physicochemical properties of the river and lake sediments, visual observation of the CLPP fingerprints showed a very little difference between the two sediment samples (Figure 4).
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