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Taken together with our findings described in this study, it appears that the possible mechanisms by which cMSCs participate in ovary repair are the inducing angiogenesis, increasing the follicular growth and improving the quality of oocytes and their ability to fertilization due to paracrine activity of administrated cells.
21 days later, control animals were intravenously injected with saline solution and experimental ones were intravenously injected with cMSCs. The results were evaluated on the 10th and 21st days after therapy (31st and 42nd days of experiment).
On the first passage, cMSCs were characterized by a lower proliferation rate, and on the 10th day, they reached only 65% of confluent versus 80% density of monolayer in cultures without cryopreservation.
Group Annexin Annexin [V.sup.+]/7[AAD.sup.-] [V.sup.-]/7[AAD.sup.-] MSCs 3.87 [+ or -] 2.63 84.08 [+ or -] 2.91 cMSCs 4.69 [+ or -] 2.16 76.16 [+ or -] 9.61 Group Annexin [V.sup.+]/7[AAD.sup.+] + annexin [V.sup.-]/7[AAD.sup.+] MSCs 12.05 [+ or -] 1.22 cMSCs 19.15 [+ or -] 7.24 TABLE 2: The average number of follicles in the ovaries.
The percentage of CD44 positive cells in MSC and cMSC suspensions was 96.21 [+ or -] 0.22% and 92.53 [+ or -] 0.24%; CD73 was 94.14 [+ or -] 0.12% and 96.98 [+ or -] 0.22%; CD105 was 97.16 [+ or -] 0.25% and 94.32 [+ or -] 0.21%, respectively.
At the same time, the morphological structure of ovaries on the 10th day after cMSC injection had a positive dynamics of development (Figures 4(c) and 4(d)).
The expert panel consisted of the members of the Nursing Research Specialty Group of the CMSC. This group initially consisted of six members, four of whom were credentialed in neuroscience, rehabilitation or advanced practice nursing and averaged eight years of experience with MS patients; a research consultant with six years of experience with MS patient research, and a research professor with 15 years of research experience with MS patients.
Data were analyzed by examining the responses separately for CMSC and nonCMSC
For Wave 1, Round 1 there were 25 CMSC nurses who identified needed research areas related to health and nursing care needs of persons with MS and their families and particular nursing interventions for which effective out comes needed to be determined.
Of the 30, 5 were affiliated with CMSC, 19 with National Multiple Sclerosis Chapter Affiliated Centers/ Clinics, and 6 with Veterans Administration Medical Centers.
Data obtained from the nonCMSC Centers were combined as one group for comparison with CMSC data.
Sixteen of the 108 research questions were rated by 25% or more of nurses from either the CMSC or nonCMSC Centers/Clinics as among the 10 most important questions.
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