CODEHOPConsensus-Degenerate Hybrid Oligonucleotide Primers
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CODEHOP offers a highly sensitive method for detecting low levels of virus while preserving the ability to detect unknown and mutated members of a virus family.
To identify the enterovirus type in the specimens, RTPCR, specific for a partial sequence of the viral protein 1 (VP1) region, was performed by using the COnsensus-DEgenerate Hybrid Oligonucleotide (CODEHOP) Primers ( (5).
Although the CODEHOP primers were elementary for rapid genotyping of the novel CVA6 strains, we identified more viruses with the designated CVA6-VP1 specific primers.