Genomic DNA sequences of the studied strains' complete ITS and the partial gene sequences of the selected 4 exons (CDC42, COXII
, HSP90, and TUB) were aligned with other Lagenidium and Pythium DNA sequences available at the National Center for Biotechnology Information by using ClustalW version 1.
To assess the impact of POPs on hepatic mitochondrial content, we measured mitochondrial DNA levels by quantitative polymerase chain reaction (qPCR), using primers specific for the COXII
gene, and determined the ratio between mitochondrial DNA and nuclear DNA as previously validated (Bonnard et al.
Overexpression of PGC-1[alpha] in skeletal muscle induces a fast (type II) to slow (type I) fiber type conversion and increases mitochondrial content and oxidative capacity through the modulation of genes involved in metabolism like Citocrome c, COXII
, and COXIV .