CPCSEACommittee for the Purpose of Control and Supervision of Experiments on Animals (India)
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Acute toxicity study was carried out for the Sesbania sesban by adapting fixed dose method of CPCSEA, OECD guidelines no 420.
All animals were handled according to the guidelines of CPCSEA, Government of India.
All the biopsy samples for MFP assay were processed within 48-72 hours by Rees' Method (11) at the Schieffelin Institute of Health Research and Leprosy Centre, Karigiri, Tamil Nadu, India, by following the national CPCSEA (Committee for the Purpose of Control and Supervision on Experiments on Animals) guidelines.
The animals were maintained as per the norms of CPC SEA and the experiments were cleared by CPCSEA and the institutional ethics committee.
The CPCSEA provides guidelines for performing experiments on animals and maintenance of animal house.
For maintaining the experimental animals, the Institutional Ethical guidelines were absolutely followed as per CPCSEA rules [Sanction No.
4,5,6) In this changing scenario, development of alternatives are the need of the day As per the recent guidelines computer simulated experiments are encouraged by CPCSEA, UGC and MCI.
All experiments on rats were carried out in accordance with the recommendation of CPCSEA guidelines for care and use of laboratory animals and the study was approved by Institutional Animal Ethics Committee.
The animals were maintained at a room temperature of 25 [+ or -] 1[degrees]C in a well-ventilated animal house and standard laboratory conditions of food and water according to CPCSEA Guidelines (14).