The enzyme reaction was measured using CPRG substrate and read at 577 nm at 1-min intervals for 20 min.
2] Nonstandard abbreviations: KLH, keyhole limpet hemocyanin; ACMIA, affinity column mediated immunoassay; WB, whole blood; CPRG, chlorophenol red-[beta]-D-galactopyranoside; CPR, chlorophenol red; LC-MS/MS, liquid chromatography-tandem mass spectrometry; LOB, limit of blank; LOD, limit of detection; LOQ, limit of quantification; MVIII, metabolite VIII.
Process of sharing CPRG information should be as open as possible
Members will discuss CPRG business with their colleagues, networks, media etc.
If CPRG member requests that an item of discussion or a meeting paper should remain confidential, this will be respected by the rest of the Group.
There is no reference in the booklet to the possibility of enlisting expert facilitation services, despite the fact that this had been suggested at a CPRG meeting ("Scene-setting, humour, and lively delivery are vital expert facilitation needed", meeting of 9 February 2001, punctuation missing in original).
It is important that the CPRG models a good process of engagement, and participation with the community, particularly with children and young people" (notes of CPRG meeting 13 October 2000).
These were the first indications that CPRG or chlorophenol red may possess estrogenic characteristics.
To prevent possible interference of CPRG, we redesigned the RS assay.
This [Beta]-galactosidase activity is quantified by the conversion of the chromogenic substrate CPRG into chlorophenol red.
The solution was prepared by adding 1 mL cycloheximide solution (20 mg/mL) and 200 [micro]L CPRG solution (10 mg/L) to 4 mL minimal medium.
After the ethanol was allowed to evaporate to dryness, 200 [micro]L medium containing CPRG
and yeast was added to each well.