CRFKCrandell-Rees Feline Kidney Cell
Copyright 1988-2018, All rights reserved.
References in periodicals archive ?
The compounds (3a-j) were also evaluated against INF A/H1N1 subtype (A/Ned/378/05), INF A/H3N2 subtype (A/HK/7/87), INF B (B/Ned/537/05) in MDCK, FCoV, FHV in CRFK, parainfluenza-3 virus, reovirus-1, sindbis virus, coxsackie B4 virus, punta toro virus in Vero, HSV-1 (KOS), HSV-2 (G), HSV-1 TK KOS ACV, vaccinia virus, VSV, in HEL and VSV, coxsackie B4 virus and RSV in HeLa cell cultures.
Bi-CR-submanifolds have an induced, generalized metric F structure and we establish the conditions for the induced structure to be CRFK. As a corollary, it follows that, if the bi-CR-submanifold is totally geodesic, the induced structure is a generalized CRFK structure.
Furthermore [5], the generalized metric F structure (G, F, F') is a generalized CRFK structure if F, F' are integrable and the eigenbundles of G, F satisfy the Courant bracket condition
Then, we will look for the conditions that characterize the case where the induced structure is a CRFK structure and we begin by the following preparations.
Then, the induced generalized metric F structure F of N is a generalized CRFK structure iff, [for all]Z [member of] [Q.sub.[+ or -]] = ker [F.sub.[+ or -]], one has
264.7 or CRFK cells viability was evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay [19].
RAW 264.7 and CRFK cells were seeded in 96well plates (2 x [10.sup.5] cells and 0.5 x [10.sup.4] cells per well, resp.) in DMEM containing 10% FBS and 1% PS.
FCV-F9 plaque assay using CRFK cells was also performed following the method described for MNV-1 and RAW 264.7 cells.
50 [iL of each mixture was then added to 6 plates of a 96-well plate containing 70% confluent CRFK cells.
A Inoculacao em cultivo celular foi realizada em celulas CRFK (Crandell-Reese Feline Kydney; ATCC, CCL-94) cultivadas com meio essencial minimo (MEM), suplementado com 5% de SBF (soro bovino fetal) e gentamicina (50mg [L.sup.-1]).
No presente estudo, as mesmas amostras utilizadas para PCR foram tambem inoculadas em cultivo de celulas CRFK. Amostras fecais com alto titulo hemaglutinante apresentaram efeito citopatogenico (ECP) 24 a 36 horas apos a inoculacao.