We performed an orthopoxvirus-specific SYBR Green (ThermoFisher Scientific, Waltham, MA, USA) real-time PCR targeting crmB
(23), and to confirm the first result, we tested all samples with an additional endpoint PCR targeting the orthopoxvirus HA gene.
The previous marketing order had been discontinued in 1994 after pressure from industry packers, says Pete Penner, chairman of the California Raisin Marketing Board (CRMB), which oversees the marketing order's activities.
Domestic raisin sales declined 3 percent a year after 1994, says Kathy Moulthrop, CRMB's communications administrator.
According to recent independent laboratory analysis sponsored by the CRMB
, a standard one-quarter cup serving of raisins contains 1.5 grams of inulin.
The viruses were almost identical, according to the partial sequence of the crmB
gene (EF612710 and FJ445748) and on the complete sequence of the hemagglutinin gene (EF612709 and FJ445747).
The name change came into effect today (Monday) and the company's common stock continue to trade under the symbol "CRMB