CSPCs were aliquoted and centrifuged to form pellets.
CSPCs were isolated from normal and degenerative human cartilage as well as rat knee joints .
The characterization of passage 1 CSPCs isolated with differential adhesion assay was analyzed.
Caption: Figure 1: Identification of CSPCs. (a) Flow cytometric analysis for CSPC surface markers.
The migration of CSPCs was documented with an inverted microscope at 0 hour, 24 hours, and 48 hours after the "scratch." The black lines indicate the baseline from which the cells migrated.
Caption: Figure 6: Pellets formed by CSPCs after 2-week chondrogenic differentiation induction.
While the ability in forming autologous cartilage is the gold standard for seed cells in the cartilage tissue engineering, cartilage stem/progenitor cell (CSPC), a subpopulation located in both normal and degenerated human joints [2-4], inspired high hopes for its in situ repairing potency.
2.5, Cytotoxicity and Proliferation Assay of LPP, Cell Counting Kit-8 (Dojindo, Japan) assay was performed in 96-well plates with different CSPC seeding density to decide optimal protocols for the following experiments.
Numbers of migrated CSPC were calculated and analyzed with the method mentioned above, Figure 4(e).
To figure out whether LPP can help in the recruitment or migration of CSPC in vivo, further studies were required.
LPP may promote the production of tissue-engineered cartilage in vitro by regulating the metabolism of CSPC, making it possible for application in transplant therapy.