In contrast, the spread and establishment of CTGV within Rondonia were evidently related to animal movement along BR 364.
The occurrence of new CTGV outbreaks in 2010 supports a nonsporadic pattern of infection in Rondonia, highlighting the establishment of this zoonotic infection within the state.
While GP2V was placed with other samples of VACV isolated from bovine outbreaks of Vaccinia virus (ARAV, CTGV
e PSTV), GP1V was placed with VACV-WR and VBH that are not associated with bovine outbreaks.
Therefore, a specific annealing site for the reverse primer is produced when these external sequences are contiguous, as occurs in CTGV (6).
Nevertheless, when we used the primers specific for CTGV detection, only CTGV and the 4 isolates were positive, generating 714-bp fragments, which indicated CTGV as the etiologic agent.
Principles associated with student-centered learning (Bonk & Cunningham, 1998; APA, 1993; CTGV
, 1992; Holmes Group; 1990), problem-based learning (Barrows, 1985, 1992) and performance assessments (Heywood, 1989; Loacker, 1991; Loacker, Cromwell, & O'Brien, 1986; Loacker & Mentkowski, 1993; Mentkowski & Loacker, 1985) form the pedagogical foundations for the SCenTRLE model.
The simulated environments that have been developed under this approach have been designed to invite the kinds of thinking that helps students develop general skills and attitudes that contribute to effective problem-solving, in addition to acquiring specific concepts and principles that allow them to think effectively about particular domains (CTGV, 1992a, CTGV, 1990).
More extended discussions of anchored instruction can be found in CTGV, 1990 and CTGV, 1992c.
These findings constitute a major distinction between CTGV (also SAV, ARAV, PSTV, and GP2V, which exhibit profiles 1 and 2) and the other Brazilian isolates BAV, VBH, and GP1V (profile 3).
In 2001, GP1V and GP2V were isolated from infected cows from adjacent farms near the area where CTGV had been found.
Regarding ha sequences, PSTV was clustered to ARAV and CTGV
, Spiro, Feltovich, Jacobson, and Coulson (1991) developed a computer-based instructional framework to combat inert knowledge by emphasizing the context of learning.