To compare the preservation of RNA using CTPM and CPM, 6 specimens were procured fresh, immediately upon surgical removal from patients.
The CTPM method reduced the processing of both fresh and fixed tissue samples to approximately 60 minutes.
Table 3 summarizes the impact of CTPM on turnaround times for our surgical pathology reports.
The integrity of extracted RNA following CTPM, particularly with addition of DEPC, was significantly improved, although 28S and 18S ribosomal bands were degraded in most samples.
1,2) This important improvement in our practice has caused no compromise in the quality of the diagnostic material, because routine histochemical and immunohistochemical stains obtained by CTPM are comparable to CPM.
Another advantage of CTPM is that it requires only about 10% of the total volume of reagents, which also are considerably less toxic than those used in CPM.