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Full-water-column abundance of sardine eggs was correlated with the abundance of eggs taken at 3-m depth with a CUFES; however direct conversion of CUFES samples to the full-water-column abundance, required by the DEPM, would add considerable variance to an estimate of daily egg production.
Using these findings as a guide, in 1997 we carried out an adaptive allocation DEPM survey in which the numbers of sardine eggs collected with the CUFES were used to determine subsequent locations of vertical net tows.
The objective of our study was to evaluate the use of the CUFES in the daily egg production method (DEPM) of estimating the spawning biomass of pelagic fishes (Lasker et al.
We used the following approach to evaluate the use of a CUFES in the estimation of daily egg production.
This survey for Pacific sardine employed a new survey design with the CUFES and the California vertical tow (CalVET, see below) (Smith et al.
This cruise was used as a standard for comparing DEPM surveys with and without the CUFES.
The CUFES was installed midship on the NOAA vessel David Starr Jordan onto the intake pipe over the side of the vessel; it extended 3 m below the water surface (see illustration in Checkley et al.
The density of eggs taken in the CUFES was expressed as the number of eggs taken per minute.
Conversion of CUFES egg density to full-water-column abundance and distribution of egg stages
Egg counts from 91 paired samples collected with the CUFES and CalVETs during leg 1 of the 1996 survey (Table 1) were used to derive a conversion factor from eggs/minute of CUFES sample to CalVET catch (R).