SYBR[R]Green qPCR was performed on DNAs extracted from seven reference strains as positive controls for the seven possible genes, including asal, GelE, cylA
, esp, hyl, VanA, and VanB.
"You want a bra your tee skims right over," Cyla
isolates from outpatients had significantly higher prevalence (p < 0.001-0.02) of the following virulence genes compared to the isolates from inpatients: agg/asa1 (58.9%/32.7%), eep (92.9%/62.8%), gelE (78.6%/60.3%), and cylA
Other virulence determinants, i.e., gelE, asa, esp and cylA
were determined in 91.6, 83.3, 25, and 16.6% of the strains, respectively.
(2011) reported a low frequency of cytolysin genes (cylA
, clyB and cylM) in water enterococci and Abrioul et al.
A trained architect whose work still stands in the Ukraine, Wiesenthal married Cyla
Muller, a girl he had known since school, in 1936.
He had planned an entirely different life when he married his high-school sweetheart, Cyla
. He studied at the Prague Technical University and looked forward to a career as an architect.
He studied in Prague and Vienna and later opened an architecture office in Lvov, Ukraine, and married Cyla
Mueller in 1936.
He was forcibly separated from his wife, Cyla
- but they were reunited after the war and remained together until her death in November 2003.
Wiesenthal had been married to wife Cyla
for only three years in 1939, when the Nazi-Soviet non-aggression pact allowed the Red Army to occupy Lvov and begin a purge of the Jewish middle class.
Wiesenthal, the wife of Nazi hunter Simon Wiesenthal, has died in Vienna aged 95.