An orthogonal analytical technique for the evaluation of charge heterogeneity is imaged capillary isoelectric focusing
Quantification of hemoglobin A1c by capillary isoelectric focusing
. Annual meeting of the American Association of Clinical Chemistry, Atlanta, Ga, 1997, 1997.
* Capillary isoelectric focusing
(CIEF) is an electrophoretic separation that employs a pH gradient within the capillary to separate zwitterons, usually proteins and peptides, based on each solutes pl--the isoelectric points of the protein samples.
In the field of Hb study, several methods have been developed, mainly based on capillary isoelectric focusing
Capillary isoelectric focusing on coated capillaries can be used to study Hb (16-21), but is slower than HPCEC (21).
This CZE assay at alkaline pH with high electro-osmotic flow seemed to provide as much resolution as some capillary isoelectric focusing assays (16,18), but not much as some others (17,19-21).
Comparison between capillary isoelectric focusing and high-performance cation-exchange chromatography for qualitative and quantitative analysis of hemoglobin variants.
Capillary electrophoresis is a new versatile analytical electrophoresis technique that uses numerous separation principles, including capillary isoelectric focusing (CIEF) [13-18].
Application of dynamic capillary isoelectric focusing to the analysis of human hemoglobin variants.
Capillary isoelectric focusing
(cIEF) was performed on a dimethylpolysiloxane (DMS)-coated fused silica capillary having an i.d.
Others have shown that capillary isoelectric focusing
with coated capillaries could also be used to separate hemoglobin variants X15-18], but only two of these publications demonstrated the possibility of Hb [A.sub.1c] determination (they did not report complete separation of Hb [A.sub.1c] from Hb [A.sub.0]) [17,18].