(redirected from Cell Death Detection ELISA)
CDDECell Death Detection ELISA (Enzyme-Linked Immuno Sorbent Assay)
CDDECommunications in Differential and Difference Equations (publication)
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The ratio of apoptotic cells to total cells was determined using the Cell Death Detection ELISA PLUS Kit.
A cell death detection ELISA kit was used to determine cytoplasmic DNA oligonucleosome fragments associated with apoptotic cell death.
Oligonucleosome fragments associated with apoptosis were quantified by cell death detection ELISA. As compared to the untreated cells, the administration of citicoline plus homotaurine demonstrated a statistically significant effect in terms of reduction of oligonucleosome levels.
Apoptotic and necrotic cell death was measured using the Cell Death Detection ELISA Kit (Roche Diagnostics, Mannheim, Germany) according to the manufacturer's instruction.
To investigate whether the liensinine and nuciferine could induce apoptosis in MDA-MB-231 and MCF-7 cells, histone-associated DNA fragments were measured in lysate (apoptosis) and culture supernatants (necrosis) of the cells treated with the various concentrations of liensinine and nuciferine for 24 h using the Cell Death Detection ELISA Kit.
RPMI-1640 medium, fetal bovine serum (FBS), penicillin, and streptomycin were purchased from Sigma (Germany), dimethylsulfoxide (DMSO) and trypan blue from Merck (Germany), trypsin/EDTA solution from Gibco (U.K), and 3-(4,5-dimetylthiazol-2-YL)-2,5-diphenyltetrazolium bromide (MTT reagent), Cell Death Detection ELISA plus kit, and In Situ Cell Death Detection Kit, POD from Roche Diagnostics GmbH (Germany) and DAB (3, 3'-diaminobenzidine).
These results were further substantiated by cell death detection ELISA. Formation of the nucleosomal DNA ladder is the hallmark of apoptosis so analysis of the pattern of DNA isolated from the cells exposed to different stress inducers was done.