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References in periodicals archive ?
The major difference in the 2 studies is between the migration patterns seen with cellulose acetate electrophoresis and IEF, although these 2 methods typically give similar migration patterns for normal and variant Hb.
The use of FPLC for diagnosis of [beta]-thalassaemias has been reported in an earlier study where the Mono Q column was used and a comparison was done with the manual cellulose acetate electrophoresis (7).
Comparison of FPLC with cellulose acetate electrophoresis for the diagnosis of [beta]-thalassaemia trait.
Cellulose acetate electrophoresis (pH 8.6) showed mild increase in the Hb A2 values for both parents with no other abnormal hemoglobin fraction.
Cellulose acetate electrophoresis and immuno-diffusion techniques.
Methodologies for Allozyme Analysis Using Cellulose Acetate Electrophoresis. Helena Laboratories.
1 shows the mobility pattern of Hb A and Hb E on cellulose acetate electrophoresis. Both the cases of HbE were from the state of Meghalaya Garos.
Table 1: Distribution of Cases According to Age and Sex Age (Years) Male Female Total Percentage 18-20 7 16 23 23% 21-30 27 28 55 55% 31-40 12 8 20 20% 41-50 1 1 2 2% Total 47 53 100 100 Table 2: Distribution of Cases showing the Hb Pattern on Cellulose Acetate Electrophoresis Hb Pattern Male Female Total Percentage Hb AA 46 52 98 98% Hb EE 1 1 2 2% TOTAL 47 53 100 100%
Although measurements of [HbA.sub.2] by cellulose acetate electrophoresis (5) and microcolumn chromatography (6) are reproducible and accurate, these methods are labour-intensive and time consuming, and automation HPLC method, with high-throughput screening was often used in routine screening of haemoglobin (7,8).
These blood samples were identified by cellulose acetate electrophoresis at alkaline pH (Helena Labs, Beaumont, Texas, USA), DEAE sepharose microcolumn (14), and HPLC (Variant, Bio-Rad Laboratories, California, USA) and these were 125 cases with normal haemoglobin ([HbA.sub.2] < 3.5%) and 95 carders [35 [beta]-thalassaemia trait ([HbA.sub.2] = 3.5-10%), 48 HbE trait (HbE = 10-40%), 7 homozygous HbE (HbE > 60%) and 5 [beta]-thal/Hb E (HbE = 40-60%)].
2 shows cellulose acetate electrophoresis of 16 of the same samples processed by the Hydrasys system.
[1] Nonstandard abbreviations: AGE, agarose gel electrophoresis; CAE, cellulose acetate electrophoresis; CZE, capillary zone electrophoresis; and IF-ES, immunofixation electrophoresis /subtraction.