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References in periodicals archive ?
Next, we performed chromatin immunoprecipitation analysis to examine in vivo association of LexA-Med6p derivatives, Med14 (for Mediator), and TBP (for transcription initiation) with a target promoter region ([8xlexA.sub.op]).
Fujii, "Efficient isolation of specific genomic regions and identification of associated proteins by engineered DNA-binding molecule-mediated chromatin immunoprecipitation (enChIP) using CRISPR," Biochemical and Biophysical Research Communications, vol.
Its binding ability was also verified by chromatin immunoprecipitation in the HCT116 cell line.
Chromatin immunoprecipitation (ChIP) analysis was performed as described previously (Jin and Felsenfeld 2006).
The results from chromatin immunoprecipitation assays indicated that Bach1 bound to the region from position -105 to -301 of the VEGF promoter in HUVECs (Figure 4(b)), which contains the consensus TCF/LEF-binding site (positions -144 to -139, Figure 4(a)), further confirming that Bach1 occupies the TCF/LEF-binding site of the VEGF promoter.
The interaction of NF-[kappa]B with the MDR1 promoter region was detected by chromatin immunoprecipitation (ChIP) assay (Fig.
7- day mouse testes were used in Chromatin immunoprecipitation for their high rate of spermatogonia.
The antibody, reportedly the industry's first, has been thoroughly validated for a number of applications including western blotting, immunofluorescence determining protein localisation, and immunoprecipitation applications such as chromatin immunoprecipitation.
Called SIF-seq, for site-specific integration fluorescence-activated cell sorting followed by sequencing, this new technique developed by researchers with the Lawrence Berkeley National Laboratory (Berkeley Lab) complements existing genomic tools, such as ChIP-seq (chromatin immunoprecipitation followed by sequencing), and offers some additional benefits.
Porvair Sciences has introduced its Chromatrap96 HT, an 96-well chromatin immunoprecipitation (ChIP) assay plate.
Chromatin immunoprecipitation (ChIP) is one such powerful technique for studying protein-DNA interactions in cells, and there are many possible ways to perform such experiments [10-12].