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Levels of proteins were measured using 20 f g of protein or equal volume of co-IP samples.
P5CR2 (Pyrroline-5-carboxylate reductase 2) and AXN1 (Axin-1) were verified by co-IP and Western blot, and no positive data was observed (data not shown).
Then, each construct was examined for interaction with the full-length DDX3 using co-IP analyses.
(e) DDX3 showed no interaction with the METTL3, METTL14, WTAP, or FTO, examined with IP and co-IP. (f) The interaction of ALKBH5 with DDX3 examined with FLAG-DDX3 IP and HA-ALKBH5 co-IP in HeLa cells.
To investigate whether BAG5 interacts with DJ-1, we performed Co-IP assay in HEK293 cells.
With Co-IP and immunofluorescence experiments, we demonstrate that BAG5 interacts with DJ-1.
Their potential interactions were further demonstrated via a Co-IP assay in C6/36 cells infected with DENV2.
For the Co-IP assay, both an enhanced green fluorescent protein (eGFP) and human influenza hemagglutinin- (HA-) tagged BiP/GRP78- overexpressing vector were constructed and transfected into C6/36 cells.
To identify the mechanism underlying PGC-1[alpha] upregulation and increased PPAR[alpha] transactivation, a series of assays, including ChIP, EMSA, and Co-IP, were conducted (Fig.
electrophoretic mobility shift assay; Chip, chromatin immunoprecipitation; Co-IP, coimmunoprecipitation.
To examine the interaction between Rheb and FKBP38, we performed a co-IP experiment with anti-Rheb, observing that Rheb and FKBP38 were bound together in eluates from cotransfected cells (Figure 1B).
Using a combination of techniques including 2-DE, co-IP, western blot, LC-MS/MS, immunofluorescence, and immunohistochemistry, we find the expression intensity of Hsp74 in bladder cancer tissues is higher than that in normal bladder tissue.