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Related to Co-immunoprecipitation: Western blot
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There are many methods, such as yeast two-hybrid, co-immunoprecipitation and so on, for determining the interaction between two proteins.
We determined the levels and functions of p53, nuclear factor-kappa B (NF-kB; a key transcriptional regulator), and mot-2 (a p53 inhibitor) and their relationships in arsenite-induced transformed HELF cells by two-dimensional electrophoresis, reverse-transcriptase polymerase chain reaction, Western blot, immunofluorescence, and co-immunoprecipitation assays.
Remnant liver tissue was homogenized and analyzed by co-immunoprecipitation and western blotting.