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coli strains were further processed for the screening of ESBL and Non-ESBL isolates by combination disc test (CDT) and MBL and Non-MBL E.
coli strains followed by Staphylococcus (17.85%) isolates, Streptococcus (13.39%) strains and gram-negative coccus (1.78%) isolates (Figure1).
coli phages candidates were prepared by incubation of purified phage with the host in LB broth supplemented with Ca[Cl.sub.2] (2 mM) for 24-48 h at 37[degrees]C with shaking, the cultures were centrifuged (6000 rpm, 10 min) then filter sterilized and the resulting crude lysate was stored at 4[degrees]C.
coli isolation and antibiotic susceptibility profiling
coli is the most ubiquitous microorganisms on Earth, existing as part of the commensally intestinal flora, where it is responsible for the production of many beneficial metabolic products.
coli isolates exhibited high rates of multidrug resistance, extended-spectrum-b-lactamase production as well as metallo-b-lactamase production (Hussain et al., 2014).
Use of the Flagellar H7 Gene as a Target in Multiplex PCR Assays and Improved Specificity in Identification of Enterohemorrhagic Escherichia coli Strains, Animal Diseases Research Institute, Agriculture and Agri-Food Canada, Canada T1J 3Z4, Canada N1G 3W4.
The epidemiology of infections caused by Escherichia coli O157:H7, other enterohemorrhagic E.
coli were isolated using EasyGel ColiScan plates (Micrology Labs, Inc., Goshen, Indiana) according to the manufacturer's instructions-For data consistency with ongoing monitoring efforts, this method was compared to Standard Methods 9222D (Feachem, 1975), the same method used in routine regulatory FC analyses, and the results of both were within 1% (data not shown).
coli isolates for resistance at the following final concentrations: ampicillin (12.5, 25, 50, and 100 [micro]g/ml), chloramphenicol (1.25.
In multivariable analysis, age less than 18 years (adjusted odds ratio 3.92; 95% confidence interval 1.43-10.68), presence of central venous catheter (adjusted odds ratio 2.12; 95% confidence interval 1.04-4.33) and exposure to piperacillin/tazobactam within 90 days prior to infection (adjusted odds ratio 2.37; 95% confidence interval 1.15-4.86) were identified as independent risk factors for acquisition of multidrug-resistant Escherichia coli bacteraemia.
Conclusion: Bloodstream infections with multidrug-resistant Escherichia coli were common in cancer patients.