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We used a standardised tail flick apparatus (May TF 0703 Tail-flick Unit, Commat, Turkey) to evaluate thermal nociception.
Adaptation Training: For the rats to have adaptation they were made to have exercise on treadmill for 10-15 min, at 20 m/min during per week (MAY TME 9805 treadmill exerciser, Commat Ltd., Ankara, Turkey).