(redirected from Cytochrome Oxidase Subunit 1)
COX1Cytochrome Oxidase Subunit 1
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We performed PCRs of the cestode-specific 12S rRNA gene (4) and cytochrome oxidase subunit 1 (cox1) (5).
Primers used were 5'-CCGGGCAAA AGTCGTAACAA-3' and 5'-ATATG CTTAAATTCAGCGGGT-3' for a region that spans the internal transcribed spacer (ITS) 1, ITS2, and 5.8S rRNA; 5'-CTACTACTAAGAATTTGCGT-3' and 5'-AATCCAAATACTTACGAG GA-3' for cytochrome oxidase subunit 1; and 5 '-CAGCGTATTGGTCCT AATAA-3' and 5 '-AGCATAAACAAA AGTAAACTCA-3' for NADH de hydrogenase subunit 1.
However, phylogenetic tree analyses of NADH dehydrogenase subunit 1 and cytochrome oxidase subunit 1 sequences showed that the clinical and Pacific cod roundworm isolates clustered with P.