D-TEST


Also found in: Medical.
AcronymDefinition
D-TESTDestructive Test
D-TESTDouble disk diffusion test (clindamycin resistance)
References in periodicals archive ?
Inducible resistance to Clindamycin was defined as blunting of the clear circular area of no growth around the Clindamycin disk on the side adjacent to the Erythromycin disk and was designated D-test positive.
This can be achieved by testing the isolates using the D-test in accordance with CLSI guidelines.
aureus meticilino resistente que se detecto en la institucion donde se realizo este trabajo y dado el impacto clinico y terapeutico que representa el aumento de las cepas sarm con resistencia agregada a la clindamicina, especialmente con la variante inducible (MLSBi), la cual presenta sensibilidad in vitro, y, aunque la resistencia inducible a clindamicina en este estudio fue baja, se debe implementar de forma obligatoria la prueba de rutina de induccion a este fenotipo por el metodo de disco (D-test) para evitar fracasos terapeuticos, la diseminacion de S.
Phenotypic Detection of Inducible Clindamycin Resistance among the Clinical Isolates of Staphylococcus aureus by Using D-Test. IJHSR.
Por lo tanto, la importancia radica en que la clindamicina podria constituir una alternativa antibiotica para SAMR tanto de la comunidad como del hospital considerando que la tecnica del D-test, para detectar la resistencia inducible, es un examen sencillo de realizar, de bajo costo, con alta sensibilidad y especificidad, y que la informacion que entrega es importante para la decision terapeutica que debe adoptar el clinico, esta tecnica se deberia implementar en todos los laboratorios de microbiologia para su uso de rutina (14).
Se empleo la prueba D o prueba de difusion de doble disco (D-test), de acuerdo a las recomendaciones (CLSI) (11).
First of all, the D-test (Martinez & Ruiz, 2011) proposed to analyse scale invariance, can only be applied when the same participant valuate the variable of interest using disparate rating scales.
Although 35% of isolates were resistant to clindamycin by single-disc testing, the D-test identified erythromycin-induced clindamycin resistance in 68 isolates, bringing the effective total figure for clinical clindamycin resistance to 62.5%.
For the D-test, erythromycin resistant samples were once again put into a Columbia broth to a concentration of 0.5-0.9 McFarland units.
The D-test for inducible clindamycin resistance was performed on 97% (112/116) of adult and 89% (125/142) of pediatric CA-MRSA isolates that were resistant to erythromycin and susceptible to clindamycin.
(15) All the erythromycin resistant and clindamycin susceptible isolates were further subjected to 'D-Test' as per the CLSI guidelines by placing an erythromycin (15[micro]g) disk at a distance of 15mm (Edge to edge) from clindamycin (2[micro]g) disk on a Mueller Hinton Agar plate previously inoculated with 0.5 McFarland bacterial suspension.
The D-test involves placing a 2-mg clindamycin disk 15-26 mm apart from a 15-mg erythromycin disk on agar that has been inoculated with the S.