D2N

AcronymDefinition
D2NDonor-to-Nonprofit (marketplace)
D2NDiablo II Network (gaming network)
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Normalized fold change Exposure (days) relative to DMSO contro C57 1 154.34 [+ or -] 20.8 2 455.09 [+ or -] 30.62 3 730.00 [+ or -] 24.00 D2N 1 1.82 [+ or -] 0.27 2 12.44 [+ or -] 2.03 3 3 39.49 [+ or -]1.20 Materials and Methods
Quantitative reverse-transcriptase PCR qRT-PCR analysis of C57 metanephri revealed time-dependent induction of cytochrome P450 1A1 (Cyplal) mRNA, whereas D2N metanephri showed a markedly abrogated response (Table 2).
To determine whether ligand binding to AHR-mediated deficits in renal cell differentiation, we examined effects of BaP exposure on D2N mice expressing the [Ahr.sup.d/d] allele and C57 mice co-treated with oe-NF, a competitive inhibitor at the AHR ligand-binding site.
C57 but not D2N metanephri exhibited decreased taurine transporter (TauT) mRNA (compare Figures 4E and 5E).
Control C57 and D2N mice showed similar numbers of glomeruli (Figure 6A).