DA-BSADopamine Conjugated with Bovine Serum Albumin (immunogen)
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Therefore, DA-bovine serum albumin (DA-BSA) and DA-ovalbumin (DA-OVA) conjugates were used as immunogen and coating antigen,respectively.
Production of mAb: Three female Balb/c mice (virus free and 6-8 weeks old) were subcutaneously injected with antigen DA-BSA. After 5 times injection, the mice serum titer of antibody (anti-DA) from the immunized mouse was determined by iELISA.
Therefore, a hapten-protein conjugate was developed and DA-BSA was injected at multipoint subcutaneously into the mice to induce the immune response in Balb/c mouse.