Sections were washed in phosphate-buffered saline-Tween and counterstained with DAPI
. Antifade mounting medium (Beyotime, Haimen, China) was added.
and PI correspond to the two fluorescent dyes used.
Then, nuclei were stained using the kit CyStain[R] UV Precise P (Partec GmbH., Munster, Germany) with 1600 [micro]l of DAPI
We used a 405-nm laser to illuminate DAPI
, a 488-nm laser for Alexa-flour (EdU), and a 594-nm laser for the natural fluorescence of Symbiodinium.
The chromosomes were counter-stained with DAPI
(0.2 mg per mL) in Vectashield Mountng Medium (Vector Laboratories, Burlingame, California, USA).
The established criteria for identification of CTCs such as DAPI
positivity, cytokeratin positivity, and CD45 negativity  demonstrate only that the isolated cells show traces of being epithelial and not of being an immune cell, but do not in and of themselves demonstrate that the cell is a cancer cell.
After 60 min, the cells were fixed with 4% paraformaldehyde and stained with DAPI
and fluorescein isothiocyanate- (FITC-) conjugated phalloidin.
HepG2 cells nuclei were stained with DAPI
Slides preparations were subjected to RNAse and Proteinase K pretreatments, followed by steps of denaturalization, probe hybridization, blocking, probe detection by means of antibodies linked to fluorochromes [anti-digoxigenin to fluorescein (FITC) and anti-biotin to rhodamine (TRITC)] (Dako, USA), washing and DAPI
staining for contrast.
Nuclei were counterstained with DAPI
Nova Lite DAPI
ANCA Kits are the first FDA cleared reagents for the detection of anti-neutrophil cytoplasmic antibodies on an automated digital IFA microscope, a long-awaited solution for laboratories performing ANCA IFA testing.
Por lo tanto, no se conoce la utilidad del bandeo DAPI
en el analisis cariotipico de las especies de Canna.