The meaning of DAPI
bands observed after C-banding and FISH procedures.
Lipid accumulation was measured on day 14 by Nile red fluorescence values normalized to DAPI
Slides preparations were subjected to RNAse and Proteinase K pretreatments, followed by steps of denaturalization, probe hybridization, blocking, probe detection by means of antibodies linked to fluorochromes [anti-digoxigenin to fluorescein (FITC) and anti-biotin to rhodamine (TRITC)] (Dako, USA), washing and DAPI
staining for contrast.
Dolayisiyla FACS ile toplanmis olan kok hucreler floresan mikroskobunda, DAPI
ile boyanmis cekirdekleri mavi renkte olan hucreler olarak goruntulendiler (Sekil 5).
showed the uniform distribution of nucleus at certain distance in the hyphae (Fig.
Nova Lite DAPI
ANCA Kits are the first FDA cleared reagents for the detection of anti-neutrophil cytoplasmic antibodies on an automated digital IFA microscope, a long-awaited solution for laboratories performing ANCA IFA testing.
Por lo tanto, no se conoce la utilidad del bandeo DAPI
en el analisis cariotipico de las especies de Canna.
After 48 h, plates were washed with PBS and cells were fixed with 4% para-formaldehyde and stained with DAPI
staining solution for 10 s.
Morphological observation of DAPI
and acridine orange/propodium iodide staining documented typical characteristics of apoptotic cell death.
The 800uL of the suspensions were removed after culture for 0 h, 4 h, 8 h, 12 h, 16 h, 20 h, 24 h, 28 h, 32 h, 36 h, 40 h and immediately poured into 2400uL of DAPI
solution (10 ug/mL, Roche Diagnostics GmbH,Germany) and kept in the dark for 10 min.
To investigate, the effects of paclitaxel on the apoptotic response, DAPI
nuclear staining was applied.
Coverslips were then washed four times with PBS for 5 minutes each and placed on 8 [micro]g/mL of the corresponding secondary antibody (Alexa Fluor-conjugated anti-rabbit IgG or anti-mouse IgG) in 5% BSA in PBS containing 300 nM DAPI