protein family and cancer," Autophagy, vol.
2- En el programa apoptotico mediante la inactivacion de DAPK
, p14, TMS1, APAF1, DIABLO, DBC1 y ASPP1.
Most frequently hypermethylated genes include COX 2, DAPK
, CDH1, CDKN2A, and hMLH1.
Koul et al., "Frequent promoter methylation of CDH1, DAPK
, RARB, and HIC1 genes in carcinoma of cervix uteri: its relationship to clinical outcome," Molecular Cancer, vol.
Slides were then incubated with DAPK
, XIAP, Bcl-2, Bax, caspase-3, Cyto c, and GAPDH antibodies, overnight in a moist chamber at 4[degrees]C, washed in PBS, and incubated with biotinylated goat anti-rat antibodies (1 : 2000, Proteintech Group).
Frequent hypermethylation of DAPK
, RARbeta, MGMT, RASSF1A and FHIT in laryngeal squamous cell carcinomas and adjacent normal mucosa.
Algunos de los genes estudiados en el contexto de hipermetilacion de sus promotores en cancer de pulmon incluyen: CDKNA/P16INK4a, RASSF1, MGMT, APC, DAPK
, FHIT, CDH13, RAR [beta], SHOX2, RUNX3, CDH1, TSCL1, ASC/TMS1, DAL1, PTEN, GSTP1.
In HNSCC, numerous studies have identified promoter DNA methylation of such genes as CDKN2A (p16), DAP kinase (DAPK
), and DNA repair genes MGMT and MLH1 (8) In comparisons of epigenetic profiles of HNSCCs, there is also evidence that epigenetic changes observed in head and neck cancer are subsite dependent.
Several methylation-regulated candidate genes have been identified in SACC, including auprabasin (SBSN) (12), aquaporin 1 (AQP1) (13), phosphatase and tensin homolog deleted on chromosome 10 (PTEN) (14), cyclin-dependent kinase inhibitors (15), RAS-associated domain family protein 1A (RASSF1) (16), and death-associated protein kinase (DAPK
) (17), but the methylation of RECK in SACC has not yet been reported.
Epigenetic modifications of DAPK
and p16 genes contribute to arsenic-induced skin lesions and nondermatological health effects.
These candidate genes include DAPK
(death-associated protein kinase 1), CDH1 [cadherin 1, type 1, E-cadherin (epithelial)], RASSF1 [Ras association (RalGDS/AF-6) domain family member 1], CDKN2A (cyclin-dependent kinase inhibitor 2A), FHIT (fragile histidine triad), MGMT (O6-methylguanine-DNA methyltransferase), and RARB (retinoic acid receptor, beta).
The critical genes regulated by Smad include the GADD45[beta]r signaling factor domain, the Bc1-2 Bim homologous factor, the death-associated protein kinase (DAPK
), the SHIP phospholipid phosphatase, and the TGF-[beta]-inducible early response gene 1 (TIEG1) .