Estandarizacion de PCR especifica de metilacion para la deteccion de metilacion en los promotores de los genes CDKN2B y DBC1
en pacientes con leucemia linfoide aguda, leucemia mieloide aguda y leucemia mieloide cronica.
Kim et al., "CK2[alpha] phosphorylates DBC1
and is involved in the progression of gastric carcinoma and predicts poor survival of gastric carcinoma patients," International Journal of Cancer, vol.
Assume that the activation rates are [k.sub.12], [k.sub.22], [k.sub.42], and [k.sub.62] for Mdm2, p53, miR-34a, and DBC1
, the degradation rates are [k.sub.13], [k.sub.24], [k.sub.43], [k.sub.52], and [k.sub.63] for Mdm2, p53, miR-34a, Sirt1, and DBC1
, the synthesis rates are [k.sub.1]1, [k.sub.21], [k.sub.4]1, [k.sub.51], and [k.sub.61] for Mdm2, p53, miR-34a, Sirt1, and DBC1
, and the translational repression rates are [k.sub.31], [k.sub.23] for p53*, p53.
More recent studies have identified frequent loss of heterozygosity in bladder SmCC at 9p21 (p16), 3p25-26 (VHL), 9q32-33 (DBC1
), and 17p13 (TP53).
Cultures were established and plants regenerated from these genetically homogeneous lines following published procedures for SEC (Wan and Lemaux, 1994), MEC (DBC1
medium for the first month, DBC2 medium thereafter; Cho et al., 1998), and SMC (Zhang et al., 1999) tissues.
In an integrated genetic/epigenetic approach, Serizawa et al (94) prospectively performed mutational screening of a set of 6 genes (FGFR3, PIK3CA, TP53, HRAS, NRAS, and KRAS) and quantitatively assessed promoter methylation status of 11 additional genes (APC, ARF, DBC1
, INK4A, RARB, RASSF1A, SFRP1, SFRP2, SFRP4, SFRP5, and WIF1) in NMI-BC tumor biopsy specimens and corresponding urine samples from 118 patients and 33 controls.
Frequent silencing of DBC1
is by genetic or epigenetic mechanisms in non-small cell lung cancers.
Nontransgenic plants were obtained from calli initiated from IEs and grown on each of five different MS (Murashige and Skoog, 1962)-based callus-induction media: (i) D medium containing 2.5 mg [L.sup.-1] 2,4-D and 0.1 [micro]M Cu[SO.sub.4] (Cho et al., 1998), (ii) DM medium containing 2.5 mg [L.sup.-1] dicamba and 0.1 [micro]M Cu[SO.sub.4] (Wan and Lemaux, 1994), (iii) DC medium containing 2.5 mg [L.sup.-1] 2,4-D and 5.0 [micro]M Cu[SO.sub.4] (Cho et al., 1998), (iv) DBC1
medium containing 2.5 mg [L.sup.-1] 2,4-D, 0.01 mg [L.sup.-1] BAP and 5.0 [micro]M Cu[SO.sub.4] (Cho et al., 1998), and (v) DBC2 medium containing 2.5 mg [L.sup.-1] 2,4-D, 0.1 mg[ L.sup.-1] BAP and 5.0 [micro]M Cu[SO.sub.4] (Cho et al., 1998).
Two putative tumor suppressor genes, deleted in bladder cancer 1 (DBC1
) and deleted in esophageal cancer 1 (DEC1), are localized on chromosomal 9q.