DD-PCRDifferential Display-Polymerase Chain Reaction
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The DD-PCR technique, developed by Liang and Pardee [18], has been widely used in plants to isolate genes that are differentially expressed in response to various stresses [21,35,19,36].
DD-PCR was performed as described in the RNAimage Kit (GenHunter Corp.).
DD-PCR was performed by means of the same protocol as described above.
Figure 1 shows examples of DD-PCR results using total RNAs isolated from leaves of drought-treated plants, and from roots and shoots of salinity-treated seedlings.
Table 2 lists the clones obtained by DD-PCR, and summarizes the homology search results.