DD-PCRDifferential Display-Polymerase Chain Reaction
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Transcriptome profiling of plants to environmental stresses can be studied using different techniques, which include DD-PCR, serial analysis of gene expression (SAGE), subtractive hybridization, DNA-chip, and cDNA microarray.
DD-PCR [18] was done using the Fermentase Kit (Clontech Laboratories, Inc.
The DD-PCR technique, developed by Liang and Pardee [18], has been widely used in plants to isolate genes that are differentially expressed in response to various stresses [21,35,19,36].