Comparative Study of Different Methods for MBL Detection (n=211) Name of Test Positive Percentage MIC 211 100 DPT 189 89.57 DDST
The MBL enzyme was not found in any of the 43 CRAb strains by the double-disk synergy test (DDST
After the evaluation of descriptive knowledge, DDST
was applied to examine the developmental levels of children.
coli isolates which were screened for ESBL production by the DDST
, then confirmed by the double-disk diffusion test (DDDT) as recommended by the CLSI.
the battalion organized a DDST
in support of the RPOE to create the first organized JTF-PO unit.
We thus evaluated two types of double disc diffusion tests (DDSTs
): imipenem- ethylene diamine tetra acetic acid (IPMEDTA) method and ceftazidime- mercapto propionic acid (CAZ-MPA) method to detect MBL production in P.
baumannii clinical isolates that were MBL producers on the basis of DDST
were detected among collections of isolates from patients hospitalized during the study period.
To perform DDST
Phenotypic method, Cefotaxime (30 [micro]g) and Ceftazidime (30 [micro]g), with and without Clavulanic acid (10 [micro]g) discs, were placed on Mueller-Hinton agar (MHA, Oxoid, United Kingdom) with 25 mm apart from each other.
Detection of ESBL producers among Gramnegative Bacteria Organism Screening Test DDST
PCDDT Klebsiella pneumoniae n=28 12 (42.8%) 12 (42.8%) 12 (42.8%) Klebsiella oxytoca n=3 1 (33.3%) 1 (33.3%) 1 (33.3%) Pseudomonas aeruginosa n=17 4 (57.1%) 4 (57.1%) 4 (57.1%) Acinetobacter baumannii n=9 3 (33.3%) 3 (33.3%) 3 (33.3%) Proteus mirabilis n=4 2 (50.0%) 2 (50.0%) 2 (50.0%) Escherichia coli n=3 2 (66.6%) 2 (66.6%) 2 (66.6%) Citrobacter koseri n=2 2 (100%) 2 (100%) 2 (100%) Chart 1.
Synergistic effect of EDTA on imipenem enhanced the inhibition zone by 8-15 mm in case of MBL positive strains while the increase for MBL negative isolates was only 1-5 mm.15 In DDST
, an imipenem (10ug) disc was placed 20mm apart (centre to centre) from a blank disc containing 10ul of 0.1 M EDTA solution.
Double-disc synergy test (DDST
) and CLSI confirmatory test were compared for the phenotypic detection of ESBL K.