DEHDDefence Estates Housing Directorate (UK)
Copyright 1988-2018, All rights reserved.
References in periodicals archive ?
Sequence analysis of the 804-bp dehD fragment from pCAMdehD: The dehD gene from pCAMdehD was confirmed by comparing its sequence with the original dehD sequence taken from the NCBI database [Accession number CAA63793.1].
PCR analysis of pCAMdehD: A PCR analysis was carried out, using primers dehDF and dehDR, to re- confirm the presence of dehD in the pCAMdehD construct.
benthamiana transformed with pCAMdehD and resistance to MCA: pCAMdehD carried both the hygromycin resistance gene and dehD, either of which could be used as a selectable marker.
dehD expression may have resulted in the degradation of MCA in the cells allowing for the development of resistant shoots and roots.
benthamiana, and the integration of dehD into the chromosomal DNA: To verify the presence of dehD, total genomic DNA samples of transformed and non- transformed 12-week-old N.
dehD expression analysis: The molecular analyses strongly indicated the integration of dehD into the chromosomal DNA of the 10 tested samples.
Thus, the leaf-painting analysis suggested that dehD was indeed expressed in N.
In this study, the GUS sequence in the pCAMBIA 1305.2 vector was replaced with dehD to develop MCA herbicide-resistant cultivars.
Thus, dehD can be useful as selectable marker in plant transformation systems.
The dehD gene fragment (798 bp) was detected in all of the independent selected lines and in the positive control pCAMdehD overexpression vector.
RT- PCR revealed that dehD was expressed in all 10 independent transgenic lines, whereas no expression occurred in non-transformed tobacco.
This indicated that dehD expression resulted in the detoxification of the herbicide within plant cells, which prevented this systemic effect.