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DEPCDiethyl Pyrocarbonate
DEPCDown East Partnership for Children
DEPCDepth Control Log (energy production)
DEPCData Evaluation and Publication Committee
DEPCDepartment of Environmental Pollution Control (various nations)
DEPCDepartment for Environment, Planning and the Countryside (UK)
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[5] Nonstandard abbreviations: miRNA, microRNA; CTC, circulating tumor cell; RBC, red blood cell; WBC, white blood cell; qPCR, quantitative PCR; FTA, Flinders Technology Associates; BD, Becton Dickinson; TR NUH-NUS, National University Hospital-National University of Singapore Tissue Repository; PBS, phosphate-buffered saline; BSA, bovine serum albumin; DAPI, 4',6-diamidino-2-phenylindole; DEPC, diethyl pyrocarbonate; [C.sub.q], threshold cycle; RT-qPCR, reverse transcription qPCR; PCA, principal component analysis; EMT, epithelial-to-mesenchymal transition.
The following reagents were added to the reaction mixture: 22.5 [micro]L of Real Master Mix/SYBR Solution, 1 [micro]L each of the upstream and downstream primers, 0.5 [micro]L of Rox reference dye, 5 [micro]L of template DNA, and 20.5 [micro]L of DEPC water.
Los controles negativos utilizados fueron la mezcla de reactivos de la PCR, que reemplazaron la muestra con agua DEPC. Las condiciones de amplificacion de la PCR fueron: 1 ciclo 95 [grados]C durante 10 min seguido por 45 ciclos de 95 [grados]C por 10 s, 63 [grados]C para GADPH, 62 [grados]C para OPG y 58 [grados]C para RANKL durante 10 s y 72 [grados]C por 30 s.
Extraction buffer 1: CTAB (2% w/v = 2 g), polyvinylpyrrolidone (2% w/v = 2 g), ethylene- diaminetetraacetic acid (25mM~ 0.74 g), tris-base (0.1M =1.2 g, pH 8.0), NaCl (1.4M =8.1 g) and 100 mL DEPC treated water.
El precipitado del RNA se disolvio en la solucion denaturizante y fue reprecipitado con isopropanol, se lavo con 70% de etanol y finalmente se disolvio en agua tratada con dietilpirocarbonato (DEPC).
Normally, diethyl pyrocarbonate (DEPC) is added to the buffers and solutions required in order to inactivate RNases, which is tantamount to overkill, or excessive prevention of secondary contamination, such as that resulting from impure chemicals or materials.
Real-time polymerase chain reaction (RT-PCR) reaction was carried out in a final volume of 25[micro]l, using 2.5[micro]l cDNA template, 12.5[micro]l SYBR Green Master Mix (Toyobo, Japan), 2.5[micro]l plus solution and 5.5[micro]l DEPC water, and 2[micro]l for forward and reverse primers (10[micro]M).
Para Trizol[R] (Invitrogen) el procesamiento se hizo teniendo en cuenta las instrucciones del fabricante; en el metodo basado en CTAB de acuerdo al protocolo propuesto por Lopez et al., (2006); y en el metodo fenol/clorofomo seguido por purificacion con columnas de SephadexG-50 considerando el protocolo sugerido por Guzman et al., (2006).Todos los extractos de RNA fueron resuspendidos en 30 [micron]l de agua tratada con DEPC y almacenados a -20[grados]C hasta su procesamiento.
Solenopsis invicta workers (100 mg) previously determined to be infected with SINV-3 were homogenized in 5 mL of diethylpyrocarbonate (DEPC)-treated water with a mortar and pestle.
In a typical GFP-siRNA transfection, SSPEI (in sterilized HEPES buffer, 20 mM, pH 7.4) and siRNA (0.5 [micro]g in DEPC water) were gently mixed at varying N/P ratios ranging from 2/1 to 16/1 and incubated at an ambient temperature for 30 min.
Determined to block the reopening of Pakistan's land routes into Afghanistan for NATO traffic under any circumstances, Saeed cobbled together an umbrella organization of 40 political and religious groups under the Difa-e Pakistan Council (DePC), Defense of Pakistan, in December.