Zygotes were divided into 3 groups: injected with Cx43 dsRNA (43 group), injected with DEPC
treated water (water group) and uninjected controls (control group).
To remove DNA from RNA samples the volume of the solution is adjusted with 1[micro]g extracted RNA and 1 [micro]l buffer DNase to a final volume of 10 [micro]l with DEPC
and incubated for 30 min at 32[degrees]C.
The RNA was subsequently precipitated by isopropanol, and the final pellet was resuspended in DEPC
(diethylpyrocarbonate) water and stored at -80[degrees]C.
The RNA pellet was resuspended in DEPC
Treated Water (Invitrogen Corp.
After globin mRNA or total liver RNA was applied for hybridization, each well was washed with DEPC
water at different temperatures, and the Yoyo-1 assay was conducted.
Lodeiro, "Review of Techniques for Characterisation of Residual Stress in Polymer Composites," NPL Report DEPC
MPR 056 (December 2006), National Physical Laboratory, Hampton Road, Teddington, Middlesex, TW11 OLW, ISSN 1744-0270.
inactivation of the recombinant enzyme showed that histidine is an important residue for catalysis.
Here we report a modified method for isolation of RNA from Dictyostelium discoideum, which avoids the usage of guanidium isothiocyanate, DEPC
and other RNase inhibitors.
2+], DMS, and DEPC
and enzymes RNase T1 and nuclease SI.
The RNA pellet was dissolved in sterile DEPC
water and the total RNA was then used for cDNA synthesis using SuperScript III[R] first strand synthesis System for RT-PCR (Invitrogen, USA).
25 mL ExScript RTase (200 U/[micro]L), and DEPC
Kwayaciiwin was set up by INAC, DEPC
and First Nation and community leaders to provide education support to Sioux Lookout-area students.