The nuclei were counterstained with hematoxylin and the sections were coverslipped with DePex
. As negative control the procedure was performed omitting the first antibody.
After serial dehydration and mounting with Depex
, the slides were examined with the aid of a Leica DMRE microscope equipped with a Leica 63 x/1.32-0.6 oil objective; images were acquired with a Leica DFC320 camera.
Las esporas se montaron en DePex
y se midio la longitud en 30 esporas de cada muestra con microscopio optico, en vista polar o ecuatorial longitudinal excluyendo el perisporio.
All slides were dehydrated in ethanol, and mounted in a synthetic resin, Depex
(Serva, Heidelberg, Germany).
Las esporas fueron montadas en DePeX
y observadas en vista polar proximal al microscopio optico de donde se obtienen datos con micrometro de tamanos longitudinales.
12(h)(3); see also, e.g., Depex
Reina 9 Partnership v.
Finally, the cells were mounted with DePeX
(SERVA Electrophoresis, Heidelberg, Germany) and the production of GHS-R was assessed with an Axioskop 2 microscope (20x, Carl Zeiss, Germany).
The sections were counterstained with haematoxylin before being dehydrated in graded alcohol and xylene and mounted with Depex
(Leica Biosystems, USA).
The otolith sections were polished with wet and dry carborundum paper (grade 1200) and mounted on microscope slides with DePeX
mounting adhesive (VWR International Ltd., Poole, England) and a cover slip.
All slides were dehydrated in ethanol and mounted in a synthetic resin (Depex
, Serva, Heidelberg, Germany).
After dehydration with ethanol (960 mL/L ethanol followed by absolute ethanol), the sections were embedded with DePeX
mounting medium "Gurr" (BDH Laboratory Supplies).
The cover slips were dehydrated in 100% ethanol and xylol before slides were cover slipped with DePex