(4) Nonstandard abbreviations: DETB, diethylthiobarbituric acid; TNM/C, ratio of total nicotine metabolites to creatinine; ACR, albumin/creatinine ratio.
We have subsequently used both the qualitative DETB extraction method and manual or automated quantitative TNM/C ratio methods to analyze >8000 urine samples in a series of smoking-related investigations .
Interestingly, no other workers appear to have attempted to use the simple DETB extraction method to identify smokers, or to evaluate its specificity and sensitivity.
The main purpose of this study was to assess the efficiency of the qualitative DETB extraction procedure and four alternative quantitative variants of the TNM method for identifying smokers in a population of diabetic patients.
The apparent smoking status of the patients was determined by the qualitative DETB extraction method as previously described , except that all the volumes used were reduced to 40% of that originally used.
More than 10 years ago, however, one of us (G.A.E.) described a simple, inexpensive qualitative colorimetric urine test for assessing smoking status based on the Konig reaction, in which pink-red chromophores formed from nicotine and its metabolites by condensation with diethylthiobarbituric acid (DETB)4 were extracted into ethyl acetate .
The performance of the qualitative DETB extraction method as judged by the cotinine-based smoking status of the patients is summarized in the first section of Table 1.
Based on the cotinine concentrations of the urine samples from the 250 subjects in our study, the specificity of the rapid and simple qualitative DETB extraction method was 100% and the sensitivity was 95%.
The panel discussion was chaired by Dheya Tawfiqi, President of Federation of Arab Engineers and CEO of his consultancy company DETB
, along with Abdul Nabi Al Sabah, the Chief Project Management Office at the Ministry of Works, Municipalities Affairs and Urban Planning and an Advisor to the Minister of Works.
The panel discussion was chaired by Dheya Tawfiqi, the President of Federation of Arab Engineers and CEO of his consultancy company DETB
, along with Abdul Nabi Al Sabah, Chief Project Management Office at the Ministry of Works, Municipalities Affairs and Urban Planning and advisor to the minister.