DGAT is a microsomal enzyme expressed in the liver and rate-limited enzyme in the process of TG synthesis, catalyzes the final step in TG synthesis by converting diacylglycerol and fatty acylcoenzyme A into TC (Farese et a).
In this study, the effects of osthol on PPAR[alpha] and PPAR[alpha]-mediated genes such as DGAT, HMG-CoA reductase, and CYP7A were investigated in vivo and in vitro.
RT-PCR was used to measure the mRNA expressions of PPAR[alpha], DGAT, HMG-CoA reductase, CYP-7A and GAPDH in mouse hepatic tissue.
2007) 5' TCAGAGGTCCCTGAACGGTG 3' DGAT (Okamoto et 5' TGGTCCCTACTATCCAGAACTCCA 3' 492 60 [degrees]C al.
To investigate whether regulation of DGAT, HMG-CoA reductase and CYP7A mRNA expressions by osthol was associated with PPAR[alpha], MK886, a specific inhibitor of PPAR[alpha], was used in the experiment (Kehrer et al.
The expression of DGAT mRNA in mouse liver tissue was significantly increased in model group (p < 0.
Effects of PPAR[alpha] inhibitor MK886 on mRNA expressions of DGAT, HMG-CoA redcutase and CYP7A in cultured BRL cells
4, after pretreatment of BRL ceils with MK886 for 2 h, the inhibition of DGAT and HMG-CoA reductase mRNA expressions by osthol was completely cancelled, but effects of MK886 itself on DGAT and HMG-CoA reductase mRNA expressions were not found.