Gel de DGGE
con los exones analizados del gen PAH en la paciente (carriles: 2, 5, 8 y 11), el padre (carriles: 1, 4, 7 y 10) y la madre (carriles: 3, 6, 9 y 12).
Las bandas mas intensas detectadas en los geles del DGGE
, las cuales indicaban las poblaciones mas abundantes en cada muestra se escindieron y la secuencia parcial del ADNr 16S de aproximadamente 500 bases se obtuvo directamente del reamplificado de las bandas.
The bacterial DGGE
image of the 16S rDNA genes extracted from initial substrate control and vermicomposts is illustrated in Figure 4.
analysis of 16S rDN A gene profiles was undertaken for (he 2006-07 and 2007-08 seasons.
Multivariate analysis of DGGE
data showed that the samples fell into two clusters along the first PCA axis on the basis of bioaugmentation treatment, and that a distinct group of samples was formed when the consortium of microorganisms (inoculum) was added, as shown in samples 2, 2R, 2A, 5, 5A, and 5R (Fig.
The 18S rDNA PCR amplicons were then separated by DGGE
choosing a gradient between 30% and 55% (urea and formamide) and 9% polyacrylamide and running conditions of 80 V for 18 h in 1XTris Acetate EDTA buffer (TAE) (10X TAE: 48.
was carried out using a CBS Scientific DGGE
system (CBS Scientific Co.
En cuanto a la amplificacion de la region 968 -- 1401 de las muestras de DNA extraido se obtuvo bandas con peso aproximado de 450 bp presentando intensidades adecuadas para el empleo de la tecnica del DGGE
For the DGGE
technique a polymerase chain reaction (PCR) was performed initially with universal primers set for bacteria based on 16S rRNA gene, U968f-GC1 and L1401.
The similarity dendrograms of bacterial 16S DGGE
fingerprints from peach orchard showed that bacterial genetic diversity in the sites under drip emitters (PIW and PCW) were discriminated from patterns of sites along the inter-rows (PID and PCD), with Pearson similarity coefficients ranging from 94.
system for comprehensive mutation screening of BRCA1 and BRCA2: application in a Dutch cancer clinic setting.
Determination of the frequency of connexin 26 mutations in inherited sensorineural deafness and carrier rates in the Tunisian population using DGGE