DHPLCdenaturing high-performance liquid chromatography
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Patients B and C, who had quite elevated electrolyte results, had extensive testing by DHPLC and full CFTR gene sequencing and despite these methods being extremely sensitive, no mutations were found.
Emmerson P, Maynard J, Jones 5, Butler R, Sampson JR, Cheadle JP Characterizing mutations in samples with low-level mosaicism by collection and analysis of DHPLC fractionated heteroduplexes.
One major drawback of DHPLC is that because of multiple amplicon melting temperatures the column temperature must be optimized for each target to achieve the optimal degree of denaturation (19).
All DHPLC results could be reproduced in a second run conducted on another day.
We identified 17 SNPs by sequencing in the screened regions of NQO1, CYP2E1, and MPO and 6 SNPs were genotyped by DHPLC in all subjects.
The long-established WAVE([R]) HS platform utilizes denaturing high-performance liquid chromatography (DHPLC); this range of systems is used throughout the world to screen for a large variety of diseases and more than 350 human genes have been screened entirely or partly by DHPLC.
We demonstrated that the WAVE DHPLC technology is capable of detecting mutant species at relative abundances as low as 2.
DHPLC (Denatured High Performance Liquid Chromatography) has been described as a highly effective means of detecting nucleotide sequence difference in PCR-amplified DNA fragments (Martin et al.
We have also shown that BLOCker-Sequencing can discriminate different viral strains which co-elute using DHPLC.
The 116 bp PCR fragment which contains the site was employed for genotyping through the DHPLC equipment.