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Then germilings were treated with 40 [micro]l of DMBQ at 10 and 20[micro]M and subsequently incubated and examined for haustorial initiation as described above.
crenata germilings resulting from seeds conditioned in water or nutrient broth medium showed similar response to DMBQ (Table 5).
crenata germlings arising from seeds conditioned in distilled water and broth medium and subsequent treated with GR24 displayed 51.9% and 43.6%, haustorium initiation in response to DMBQ respectively (Table 6).
Effects of carbohydrates on haustorial initiation in response to DMBQ:
DMBQ (10 and 20 pM), applied to Striga germilings resulting from seeds conditioned in water displayed 64 and 71 % haustorium initiation, respectively (Table 5).
DMBQ applied to Striga germilings resulted from seeds previously conditioned in water and GR24 induced 70% haustoria (Figure 10).
Haustorium initiation in response to DMBQ was inhibited by the higher level of salt.
Results showed that Striga germling resulting from seeds conditioned in water and /or nutrient broth medium showed similar response to DMBQ. However, haustorium initiation by the root macerate was significantly reduced.
Striga germlings resulting from seeds conditioned in water were more responsive to DMBQ than those conditioned in the nutrient broth medium.
The high MHD values may reflect a varied cell wall composition in these sorghums that when digested with peroxidases release a more active or diffusible xenognosic compound than DMBQ. The low MHD sorghums may possess traits that interfere with [H.sub.2][O.sub.2] production, have lower levels of peroxidases, or altered root epidermal cell wall structure such that the xenognostic quinones are not produced.
Abbreviations: DMBQ, 2.6-dimethoxy-l,4-benzoquinone; MGD, maximum germination distance; MHD, maximum haustorial distance.
The PSII activity was measured with DMBQ as electron acceptor in 60 mM NaCl, 4 mM Mg[Cl.sub.2], 40 mM Tricine-KOH (pH 8,0), 2 [micro]M gramicidin, 2.0 mM potassium ferricyanide, and 0.12 mM DMBQ.
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