This final preparation included DMEM
medium with 5% CSF (20).
Caco-2 cells were grown at 37[degrees]C in an atmosphere of 5% C[O.sub.2] in Dulbecco's Modified Eagle Medium (DMEM
) supplemented with 10% fetal bovine serum.
After 6 hrs transfection, the culture medium was replaced with fresh DMEM
supplemented with 10% FBS, and cells were cultured for 24 hrs.
For example, DMEM
contains approximately 40 [micro]M phenol red, while DMEM/F12 and RPMI 1640 contain approximately 22 [micro]M and 13 [micro]M, respectively (2) (Wesierska-Gadek et al., 2007).
The cells were seeded at a density of 3 x [10.sup.4] cells/well in a DMEM
culture medium supplemented with 10% FBS in 96-well plates and incubated for overnight.
Measurement of Hydrodynamic Radii of [Pr.sup.3+]:La[F.sub.3] ([C.sub.Pr] = 7%) NPs and/or Their Agglomerations in Distilled Water, DMEM
, and RPMI-1640 Biological Mediums.
Briefly, IM-BAT cells were seeded to be 60-80% confluent at the time of transfection in DMEM
:F12 medium containing 10% FBS without antibiotics.
We changed the complete culture medium into a 10 ml serum-free high-glucose DMEM
and cultured for another 24 hours.
6 x[10.sup.5] cells were seeded in 35 mm cell culture dishes in DMEM
medium containing 10% FBS and 1% PenStrep.
Then, 3ml of Plasmid liposome complex was added into cell dish and incubated at 37AdegC in 5% CO2 for 6h, after which we removed the primary medium and replaced it with fresh DMEM
with 10% FBS.
Osteogenic medium consisted of DMEM
enriched with 10mg/ml ascorbic 2-phosphate (Sigma, USA), 10nM Dexamethasone (Sigma, USA), 10 mM B-Glicerol phosphate (Sigma, USA).
After the first enzyme digestion, testicular tissue had been digested to smaller pieces and were pipetted for ten min, then centrifuged at 120 g for 5 min and resuspended in 3 ml of DMEM
(repeated three times).