Ionizing radiation induces EGFR internalization to nucleus, association of EGFR with DNA-PK
, enhancing kinase activity of the DNA-PK
complex and increasing the repair of DNA strand breaks (14).
Merck will combine the assets with its existing DNA-PK
assets into a single development program.
All components of the DNA-PK
were found in the radiosensitive spermatogonia, and also, it is demonstrated that Ku ensures the fidelity of spermatogenesis so that the Ku70 and/or Ku80-deficient testis displays elevated levels of DSBs as well as enhanced apoptosis and reduced sperm production [12, 14].
Antibodies for DNA-PK
, p-ATM, p-ATR, p-Chk1, p-Chk2, p-H2AX, p-P53, Ku80, SOD1, catalase, GPX1, and beta-actin were purchased from Cell Signaling Technology (Danvers, MA, USA).
Interaction of the doublestrand break repair kinase DNA-PK
and estrogen receptor-alpha.
Several studies have shown that DNA-PK
phosphorylates XRCC4 in vitro, decreasing its interaction with DNA, although the significance of these phenomena is presently unclear.
normally coordinates the repair of simultaneous breaks in both strands of molecules that comprise DNA.
Nevertheless, the scientists discovered, the DNA breaks during HIV integration surprisingly activate DNA-PK
, which then performs an unusually destructive role: eliciting a signal that causes the CD4+ T cell to die.
But the new treatment has been found to attack and destroy DNA-PK
Treatment with wortmannin (1 and 5 [micro]M), an inhibitor of DNA-PK
and ATM (Sarkaria et al.
The DNA-dependent protein kinase, DNA-PK
, was first detected in the nuclei of mammalian cells (Walker et al., 1985; Carter et al., 1990).
In addition, vanillin is able to block DNA repair by nonhomologous DNA end-joining (NHEJ) and to selectively inhibit DNA-protein kinase (DNA-PK
) activity in experiments using human lymphoma GM00558 cells and related gene deficient cells.