Among different chaperone plasmids checked by co-expression, the pKJE7 plasmid set containing DnaK
, DnaJ, GrpE chaperone genes demonstrated higher soluble yield in comparison to pGro-7, pG-KJE8, pTf16 and pG-Tf2 plasmids.
Chamberlain, "Role for dnaK
locus in tolerance of multiple stresses in Staphylococcus aureus," Microbiology, vol.
The cells remained untreated (medium control) or were exposed to 10 ng/ml lipopolysaccharide (LPS) (Escherichia coli O26:B6, Sigma-Aldrich Chemie GmbH) or to various concentrations of recombinant DnaK
(Stressgen, Victoria, BC, Canada), 13mer peptides derived from the proinsulin molecule  (IHB, Leiden University Medical Center, Peptide Synthesis Facility, Leiden, The Netherlands) (Table 1), or to mixtures of DnaK
Cassataro, "Vaccination with Brucella recombinant DnaK
and SurA proteins induces protection against Brucella abortus infection in BALB/c mice," Vaccine, vol.
Four genes (groEL1, inf B, dnaK
, and leuA) were selected for primer design; they are located at different genomic regions of bacteria (Figure 1).
Bukau, "Trigger factor and DnaK
cooperate in folding of newly synthesized proteins," Nature, vol.
coli dnaK-minus strain was used to eliminate endogenous DnaK
, the bacterial homologue of Hsp70.
The crystal structure of the Dnak
peptide binding domain has confirmed that hydrophobic polypeptide of at least seven residues are optimal binding partners.
Even more, other proteins, such as glycine cleavage system protein H, isocaprenoylCoA:2-hydroxyisocaproate CoA-transferase, and molecular chaperone DnaK
, that have not been previously cited in proteomic studies were also detected in lower but significant amounts.
Rhizobial 16S rRna and dnak
genes: mosaicism and the uncertain phylogenetic placement of Rhizobium galegae Applied Environmental Microbiology, v.71, n..3, p.13281335, 2005.
Identified protein Accession Score EGCg number change (a) Response to stress, cellular processes: detoxification 1 Molecular chaperone ABR75475.1 123 DnaK
[up arrow] 2 Chaperonin GroEL ABR79862.1 85 [up arrow] 9 Alkyl hydroperoxide ABR76100.1 119 reductase, AhpC [up arrow] 12 Superoxide dismutase.
The dksA gene function was first identified as a dose-dependent multicopy suppressor of temperature sensitivity and filamentation phenotype of a dnaK
gene deleted mutant of E.