DNBS led to an enhanced expression of mRNA of IL-12 and aggravated colon injury.
Significant upregulation of IFN-[gamma] mRNA expressions in the DNBS model group, which was attenuated by 5-ASA and CHCR as seen by their levels of their transcripts, seems favorable (table 2).
50% ethanol) used for dissolving DNBS showed results similar to those in the normal control animals.
The DNBS model closely mimics the modifications observed in the colonic epithelium of patients with IBD.
The intestinal inflammatory process induced by DNBS in mice was also characterized by an altered expression of the different colonic markers evaluated.
9% protopine, used as analytical markers, possess anti-inflammatory effect in the DNBS experimental model of mouse colitis, a well characterized model of colonic transmural inflammation similarly to what occurs in human Crohn's disease (Martin et al.
Abbreviations: AFC, total alkaloid fraction extracted from the aerial parts of Fumaria capreolata: ANOVA, one-way analysis of variance; BPC, base peak chromatogram; DAD, diode array detection; DNBS, dinitrobenzenesulphonic acid; EIC, extracted ion chromatogram; ESI, electrospray ionization; GAPDH, 3-phosphate dehydrogenase; 1BD, inflammatory bowel disease; ICAM, intercellular adhesion molecule; IFN-[gamma], interferon-[gamma]; IL, interleukin; iNOS, inducible nitric oxide synthase; LC, liquid chromatography; LPS, lipopolysaccharide; MMP, metalloproteinase; MS, mass spectrometry; RT, retention time; TGF, transforming growth factor; TNF[alpha], tumour necrosis factor [alpha]: TOF, time-of-flight; ZO, zonula occludens.
In current work, two-dimensional difference in gel electrophoresis (2D DIGE) was performed to screen the potential protein targets in HaCaT responding to DNBS stimulation.
RNA isolation and real-time PCR: We performed real-time PCR (qRT-PCR) to detect the mRNA expression levels of the corresponding genes of the 8 identified proteins most differentially expressed in HaCaT due to DNBS stimulations.
Grounded on DeCyder analysis, the ratios of normalized spot intensities of DNBS treated HaCaTto untreated HaCaT cells were calculated.