DNCBDomiciliary Nursing Care Benefit (Australia)
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Cells were then seeded into 96 well plates at 1.6x[10.sup.5] cells/well and dosed with proficiency substances solubilized in the appropriate solvent over an 8-dose range, DNCB positive control (8 [micro]g/ml), DMSO solvent control and RPMI culture medium solvent control.
An AD mouse model was generated by treatment of the NC/Nga mice with DNCB, as previously described with a slight modification [41].
1 day after treatment with colchicine), a sensitizing dose of 2% DNCB about 0.1ml dissolved in acetone was applied on the right side of skin of thorax region below arm of each mice in treated groups.
Methods: We induced AD-like inflammation by application of DNCB on the dorsal skin of female skh-1 hairless mice.
For characterization of dermatitis model, rats were randomly assigned to two different groups (5 rats per group): control and DNCB sensitized.
Cell mediated immune response (Mean+-SE) to Dinitrochlorobenzene (DNCB): In vivo Dinitrochlorobenzene (DNCB) test was used to examine the delayed-type hypersensitivity reaction in broiler chickens of all groups (mm+-SE) at 24 and 72 h post DNCB injection.
Ketoprofen at 1mg/kg, and 5mg/kg suppressed the DTH response 24hrs after the application of DNCB challenge.
Dinitrochlorobenzene (DNCB) was the first sensitizer used for the treatment of AA.48 Other contact sensitizers used in alopecia areata are diphenyl-cyclo-propenone (DPCP) and squaric acid dibutyl ester (SADBE).49 The only disadvantage of DNCB is its mutagenicity by Ames test.
Later, potent contact allergens namely dinitrochlorobenzene (DNCB) and diphenylcyclopropenone (DPCP) replaced the allergens that were used earlier (33).