Cells were then seeded into 96 well plates at 1.6x[10.sup.5] cells/well and dosed with proficiency substances solubilized in the appropriate solvent over an 8-dose range, DNCB
positive control (8 [micro]g/ml), DMSO solvent control and RPMI culture medium solvent control.
An AD mouse model was generated by treatment of the NC/Nga mice with DNCB
, as previously described with a slight modification .
1 day after treatment with colchicine), a sensitizing dose of 2% DNCB
about 0.1ml dissolved in acetone was applied on the right side of skin of thorax region below arm of each mice in treated groups.
Methods: We induced AD-like inflammation by application of DNCB
on the dorsal skin of female skh-1 hairless mice.
For characterization of dermatitis model, rats were randomly assigned to two different groups (5 rats per group): control and DNCB
Preparation of 1-Chloro-2,4-dinitrobenzene Preparation (DNCB
Cell mediated immune response (Mean+-SE) to Dinitrochlorobenzene (DNCB
): In vivo Dinitrochlorobenzene (DNCB
) test was used to examine the delayed-type hypersensitivity reaction in broiler chickens of all groups (mm+-SE) at 24 and 72 h post DNCB
Haptens, such as l-chloro-2, 4-dinitrobenzene (DNCB
), can induce DC maturation.
Ketoprofen at 1mg/kg, and 5mg/kg suppressed the DTH response 24hrs after the application of DNCB
) was the first sensitizer used for the treatment of AA.48 Other contact sensitizers used in alopecia areata are diphenyl-cyclo-propenone (DPCP) and squaric acid dibutyl ester (SADBE).49 The only disadvantage of DNCB
is its mutagenicity by Ames test.
Later, potent contact allergens namely dinitrochlorobenzene (DNCB
) and diphenylcyclopropenone (DPCP) replaced the allergens that were used earlier (33).