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The principle was based on the reaction of Ellman's reagent (5,5'-dithiobis (2-nitrobenzoic acid) or DNTB) with the thiol group of GSH at pH 8.0 to produce 5-thiol-2-nitrobenzoate which is yellow at 412 nm.
Aliquots of the incubation mixture were transfered to the DNTB solution and the optical density read at 412 nm.
The Vibrosort DNTF separator, the Royselect DNTB paddy separator and the indented cylinder are other important system components.
Disodium salt, hexahydrate and guanidine hydrochloride, ultrapure water, 5,5-dithiobis-2-nitrobenzoic acid (DNTB), and trichloroacetic acid were purchased from Research Organics, 4353 East 49th Street, Cleveland, Ohio 44125; superoxide dismutase, glutathione peroxidase, glutathione reductase, glucose-6-phosphate dehydrogenase, catalase, total cholesterol, triglyceride, and HDL-cholesterol assay kits were purchased from Randox Laboratories Co., Antrim, UK.
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