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Related to DNase: Dnase 1
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5 [micro]L each of 10 [micro]M forward and reverse primers, 2 [micro]L DNase and RNase-free water.
RNA was extracted using TRIzol[R] reagent (Life Technologies) followed by DNase 1 (Thermo Fisher Scientific, Waltham, MA, USA) digestion according to the manufacturer's protocols.
RNA preparations from AcSINV-3 (2 replicates, Ci and Di) were treated with DNase I, reverse transcribed, and amplified by qPCR.
Specifically, the scientists investigated the role of extracellular DNases (eDNases) produced by C.
The results in table 3 and 4 showed a significant increase in both acid and alkaline DNase activity and specific activity in sera group A [Iraqi workers employed on the operation of diesel generators] when compared to group B [control group] (p<0.
In the present study, the chitosan-DNA nanoparticles displayed resistance against DNase I degradation (Figure 2).
RNA cleanup was performed if samples were contaminated with DNA by treating the RNA preparation with an additional DNase I digestion and, if required, a final cleanup using the RNeasy Mini Kit (Qiagen).
Dornase alfa is a recombinant form of the human DNase I enzyme, which digests extracellular DNA material.
Measurement of unpolymerized (G- actin) and total actin was done by DNase inhibition assay [30] with slight modifications [7] to stabilize the inhibitory activity of the platelet lysate.
Future studies will include additional chromatin analysis including DNase I hypersensitivity in an effort to further describe chromatin structure in the MLL bcr.
CDT-B has a DNase like activity (9-11); it blocks cell cycle progression by activation of a DNA damage checkpoint (12, 13) that eventually leads to apoptosis (14-15).
Additional reagents needed are amylase, DNase, RNase, protease, and Luria Bertani plates with the appropriate antibiotic.