Immediately, before the introduction, the cells were washed with a Dulbecco's phosphate-buffered saline (DPBS
, Paneco, Russia).
Parthenogenetic-activated oocytes were washed twice with 0.4% (w/v) BSA-supplemented DPBS
(Welgene, Korea), followed by a 4 h incubation in PZM-3-containing 2 mmol/L 6-dimethylaminopurin for post-activation.
The seeded scaffolds were fixed in 2% glutaraldehyde in DPBS
, dehydrated over an ascending propanol concentration, critical point dried (in-house designed system) and sputter coated with gold sputter coater (Polaron range, SC7620).
The condensed GSE was prepared in DPBS
solution (Sigma) and sterile filtered (a pore size = 0.45 [micro]m, Corning, USA) for future use.
The ability of epidemiologic studies to address these health questions has been seriously limited by inadequate individual assessment of exposure to DPBs
For the experiments cells were washed twice with DPBS
and diluted with deionized and distilled water to a final concentration of 2.5 x [10.sup.8] [+ or -] 9 x [10.sup.5] cells/L.
COCs were denuded of cumulus cells by vortexing in the presence of 0.1% hyaluronidase in DPBS
. The denuded oocytes were cultured in tissue culture medium (TCM) 199 containing 10 [micro]g/mL Hoechst 33,342 for 30 min in humidified air.
The cells were collected using StemPro Accutase (Life Technologies) washed with DPBS
and incubated for 1 hour with the following antibodies: anti-CD14/Brilliant Violet 605, anti-CD16/Brilliant Violet 421, anti-CD64/Alexa Fluor 488, anti-CD163/Alexa Fluor 647, and anti-CD200R/Phycoerythrine (PE) (all from BioLegend, USA).
In order to minimize this systematic error, serum concentrations were normalized concentration within our samples to 4.5 g/dL, by dilution with Dulbecco's phosphate-buffered saline (DPBS
) containing [Ca.sup.2+] and [Mg.sup.2+] (Life Technologies, Darmstadt, Germany).
Ovaries were harvested once a week for one year from Holstein cows from a local abattoir, trimmed of adhering tissue and transported to the laboratory in Dulbecco's phosphate buffered saline (DPBS
) at a temperature ranging from 34[degrees]C to 37[degrees]C within 2 h post slaughtering.
ZTBL disbursed Rs 37.9 billion or 54 per cent of its targets and Domestic Private Banks (DPBs
) disbursed Rs 37.3 billion or 69 per cent of their targets.
gingivalis viable cultures used for the scratch assay were washed twice with Dulbecco's DPBS
(Invitrogen) and resuspended in keratinocyte serum-free medium (SFM, Invitrogen) at the required OD690.