2012), we queried whether DPHD would replicate effects of E2 on cell survival or proliferation.
Effect of DPHD on nontransforined osteoblast cell differentiation and maturation
Estrogen and all concentrations of DPHD promoted alkaline-phosphatase expression in osteoblasts compared to controls (p <0.
Effect of DPHD on expression of characteristic osteoblast mRNAs
2] and DPHD in Erk1/2 response, cell proliferation, and ALP expression, it was likely that quantitative differences in specific transcriptional targets exist.
Unique effects of DPHD on bone matrix production and on osteoclast regulatory proteins
To evaluate the effect of DPHD on terminal differentiation of nontransformed osteoblasts, we evaluated mineralization of osteoblast-produced bone matrix using von Kossa silver staining at 21 days.
DPHD promoted proliferation, differentiation, and reduced production of RANKL relative to OPG at concentrations as low as 10 nM.
The effects of DPHD on human osteoblasts were similar to those of [E.
2] and DPHD increased OPG and suppressed RANKL, but DPHD gave a dose response for RANKL mRNA that extended well beyond the effect of [E.
Here, we show that DPHD at concentrations as low as 10 nM positively affects the differentiation in nontransformed human osteoblasts.
The direct estrogen action stimulating Erk1/2 was clearly seen in the h-OB cells and this effect was particularly strongly stimulated by DPHD (Fig.