Moreover, the scavenging activity of the extracts was measured using DPPH
, hydrogen peroxide, and reducing power.
Temperature, pH, and E/S ratio were selected as the independent variables and analyzed according to the preliminary experiment results, whereas DPPH
free radical scavenging activity was selected as the dependent variable.
radical-scavenging = [(Absorbance of control - Absorbance of sample) / Absorbance of control] X 100
In the antiradical scavenging property test the extract showed at 64.1 ug/ml 50% DPPH
radical scavenging activity and in the MTT assay the extract has relatively low cytotoxicity where the IC50 value of extract against Vero cells was 1078.69 ug/ml.
Antioxidant activity of ethyl haematommate, ethyl orsellinate, and brialmontin 1 is not reported, whereas zeorin has not shown potent antioxidant activities in DPPH
, SOI, NO, and metal chelating assays .
The antioxidant assays chosen for this study are 1,1-diphenyl-2-picrylhydrazyl (DPPH
), 2,2-azino-bis-(3- ethylbenzothiazoline-6-sulfonic acid) (ABTS), and ferric ion reducing antioxidant power (FRAP) assays which were used to measure the relative antioxidant ability to scavenge the free radicals produced in the reagents.
, 95%) was purchased from "Sigma-Aldrich" (St.
The concentration of Zn, Fe and total antioxidant capacity determined by DPPH
method declined with longer polishing, while FRAP method was not detected the capacity after 15 s polishing.
and KBr were attained from Aldrich Chemical Co.
where; [A.sub.0], [A.sub.1] and [A.sub.2] are absorbance of DPPH
without sample, sample and DPPH
, and sample without DPPH
The antioxidant activity of ethanolic extract of sugar beet powder and cookies was determined based on the radical scavenging ability in reacting with a stable DPPH
free radical (Mohdaly et al.,2010; Sakac et al., 2010).